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Titlebook: Cardiac Cell and Gene Transfer; Joseph M. Metzger (Director) Book 2003 Humana Press 2003

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书目名称Cardiac Cell and Gene Transfer
编辑Joseph M. Metzger (Director)
视频video
概述Includes supplementary material:
丛书名称Methods in Molecular Biology
图书封面Titlebook: Cardiac Cell and Gene Transfer;  Joseph M. Metzger (Director) Book 2003 Humana Press 2003
描述Heart disease is the leading cause of death in developed countries. Recent experimental advances featuring cellular, molecular, and genetic tools and technologies offer the potential for new therapeutic strategies directed toward remediation of inherited and acquired heart diseases. Whether these recent basic science advances will ultimately translate to clinical efficacy for patients with heart disease is unknown and is important to ascertain. Cardiac Cell and Gene Transfer: Principles, Protocols, and Applications is designed to provide the reader with up-to-date coverage of a myriad of specific methodo- gies and protocols for gene and cell transfer to the myocardium. Each chapter features a “Notes” section that provides useful “how to” problem-solving insights that are often left unstated in standard published protocols. Cardiac Cell and Gene Transfer: Principles, Protocols, and Appli- tions addresses principles and applications of cell and gene transfer to the heart, including protocols for vector production and purification. Detailed step-by-step methods and applications for first/second-generation adenoviral vectors, adeno-associated vectors, gutted adenoviral vectors, and len
出版日期Book 2003
版次1
doihttps://doi.org/10.1385/159259350X
isbn_softcover978-1-61737-292-6
isbn_ebook978-1-59259-350-7Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightHumana Press 2003
The information of publication is updating

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Bashir Ahmad,Sotiris K. Ntouyases for the investigation of cardiac physiology. Methods include morselization or perfusion of the myocardium with various enzymatic solutions to disperse cells. Similar methods have been translated to larger species with variable yield and viability.
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Lentivirus Vector-Mediated Gene Transfer to Cardiomyocyteshe roles of individual genes in cardiovascular pathophysiology. Both efficient delivery and long-term expression of transduced genes is required before the full benefit of genetic manipulation strategies can be realized in the cardiovascular system. However, all the current methods of gene delivery have major limitations.
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Suppressor tRNAs in vivo can be regulated by the presence or absence of a suppressor tRNA. This technique has provided new approaches to cancer therapy (.) as well as to the study of visual system development in . (.).
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Antisense OligonucleotidesA (mRNA). By binding to the mRNA, the oligonucleotide prevents protein translation. This blockade may be mediated by RNAase-H degradation of the RNA-oligonucleotide complex. In addition to negative effects on protein translation, oligonucleotides may inhibit mRNA transcription through triple helix formation with complementary DNA regions (.–.).
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Book 2003technologies offer the potential for new therapeutic strategies directed toward remediation of inherited and acquired heart diseases. Whether these recent basic science advances will ultimately translate to clinical efficacy for patients with heart disease is unknown and is important to ascertain. C
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