Titlebook: Cancer Stem Cells; Methods and Protocol Gianpaolo Papaccio,Vincenzo Desiderio Book 2018 Springer Science+Business Media LLC 2018 Tumor type

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Runtime Web-Service Workflow Optimizationred the cause of tumor progression and recurrence after conventional therapies, based on their enhanced therapy resistance properties. A method commonly used to assess CSC potential in vitro is the so-called tumor spheres assay in which cells are plated under non-adherent culture conditions in serum

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International Year of Planet Earthdentify genetic determinants of drug resistance versus sensitivity, and to study new stem cell-directed therapies. Here, we describe our standardized procedure for in vitro drug screening on colorectal CSCs, taking irinotecan as an example.

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International Year of Planet Earthng the importance of cancer stem cells in tumor reoccurrence after radiotherapy. Literature is replete with experimental and clinical evidence that the cancer stem cell population in a tumor affects its radiocurability. An important consideration for radiotherapy is the microenvironmental stimuli in

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Cindy Smart,Arthur Grimes,Wilbur Townsendr changes leading to their trans-differentiation into mesenchymal cancer cells that are capable of migration, invasion, and metastasis to other tissues and organs. Here, we present a method for in vitro induction of EMT in prostate cancer cell lines using lentiviral expression of a PMLI isoform muta

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Cindy Smart,Arthur Grimes,Wilbur Townsendogical mechanism in malignancies and defining of molecular biomarkers to monitor disease status. The most attractive advantage of microarray technology is its application to simultaneous analysis of miRNA expression pattern with a large amount of assessments. In this chapter, we provide a facile and