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Titlebook: Cancer Stem Cells; Methods and Protocol Gianpaolo Papaccio,Vincenzo Desiderio Book 2018 Springer Science+Business Media LLC 2018 Tumor type

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发表于 2025-3-21 20:08:46 | 显示全部楼层 |阅读模式
书目名称Cancer Stem Cells
副标题Methods and Protocol
编辑Gianpaolo Papaccio,Vincenzo Desiderio
视频video
概述Includes cutting-edge techniques for the study of cancer stem cells.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Cancer Stem Cells; Methods and Protocol Gianpaolo Papaccio,Vincenzo Desiderio Book 2018 Springer Science+Business Media LLC 2018 Tumor type
描述This detailed volume gathers a comprehensive collection of methods, protocols, and procedures used for the identification, characterization, and selection of cancer stem cells. These include surface marker expression, side population, spheres formation, ALDH activity, and numerous others. Written for the highly successful .Methods in Molecular Biology. series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and expert tips and troubleshooting advice to avoid potential pitfalls. .Comprehensive and practical, .Cancer Stem Cells: Methods and Protocols. provides researchers with the techniques they will need in order to pursue vital research into this complex field of study. .
出版日期Book 2018
关键词Tumor types; Cancer therapy; CSCs; Cellular plasticity; Tumorigenic assays
版次1
doihttps://doi.org/10.1007/978-1-4939-7401-6
isbn_softcover978-1-4939-8474-9
isbn_ebook978-1-4939-7401-6Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media LLC 2018
The information of publication is updating

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发表于 2025-3-22 00:13:15 | 显示全部楼层
https://doi.org/10.1007/978-3-642-19294-4rates that cancer stem cells can survive and generate colony growth in an anchorage-independent culture model. The 0.005% crystal violet solution is used in this assay to visualize the generated colonies.
发表于 2025-3-22 02:01:49 | 显示全部楼层
https://doi.org/10.1007/978-4-431-76428-1ion of the explain immunohistochemical methods used in the characterization of CSCs. Furthermore, focus on the most common troubleshooting in CSCs IHC is provided, especially the pitfalls of the CSCs markers IHC on tissue microarrays.
发表于 2025-3-22 05:20:34 | 显示全部楼层
International Year of Planet Earthand other cell types that prevail in the tumor milieu. In this chapter, we have described the methodology to develop in vitro 3D tumor models that incorporate these microenvironmental characteristics and design experiments that generate endpoints for understanding radioresistance in cancer stem cells.
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Book 2018tion of cancer stem cells. These include surface marker expression, side population, spheres formation, ALDH activity, and numerous others. Written for the highly successful .Methods in Molecular Biology. series, chapters include introductions to their respective topics, lists of the necessary mater
发表于 2025-3-22 18:39:53 | 显示全部楼层
In Vitro Tumorigenic Assay: The Tumor Spheres Assay,-free medium supplemented with growth factors. Tumor spheres assays have been used in cancer research as an intermediate in vitro cell culture model to be explored before performing more laborious in vivo tumor xenograft assays.
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发表于 2025-3-23 03:37:56 | 显示全部楼层
Immunohistochemistry for Cancer Stem Cells Detection: Principles and Methods,ion of the explain immunohistochemical methods used in the characterization of CSCs. Furthermore, focus on the most common troubleshooting in CSCs IHC is provided, especially the pitfalls of the CSCs markers IHC on tissue microarrays.
发表于 2025-3-23 09:20:41 | 显示全部楼层
Book 2018ials and reagents, step-by-step, readily reproducible protocols, and expert tips and troubleshooting advice to avoid potential pitfalls. .Comprehensive and practical, .Cancer Stem Cells: Methods and Protocols. provides researchers with the techniques they will need in order to pursue vital research into this complex field of study. .
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