Titlebook: Calcium signalling; Approaches and Findi Lars Kaestner Book 2013 Springer-Verlag Berlin Heidelberg 2013 calcium signalling.cardiac myocytes

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https://doi.org/10.1007/978-3-662-32527-8 the application of a calcium ionophore, such as A23187or ionomycin, to provoke an intracellular situation similar to the digital approach discussed in chapter 2 - Direct evidence - the digital approach. An even more elegant approach is to induce a transient disturbance in cardiomyocytes, the applic

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Grundsatz der Entgeltgleichheit im AGG,alcium signalling has not been written. Although certain effects of calcium in red blood cells have been known for more than 50 years, the means by which calcium enters the red blood cells still has not been completely elucidated. However, what has been clear for quite some time is how the calcium i

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https://doi.org/10.1007/978-3-662-57449-2is far from being completely understood. Therefore further basic research is necessary. With recent developments of optical technologies novel tools to investigate cardiac physiology and pathophysiology became available. They comprise non-linear imaging techniques such as second harmonic generation

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https://doi.org/10.1007/978-3-322-94190-9ent screens. This starts with an optimised culture of human adult cardiomyocytes, allowing culture with diminished dedifferentiation for one week. To allow fluorescence based molecular imaging genetically encoded biosensors need to be expressed in the cardiomyocytes. For transduction of end-differen

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https://doi.org/10.1007/978-3-322-85987-7matic because of the autofluorescence or absorption properties of the intracellular milieu. Here, we describe experiments to assess the usability of popular calcium indicators such as Fura-2, Indo-1 and Fluo-4. In our experiments, Fluo-4 turned out to be the preferable indicator because (i) its exci