Titlebook: Calcium signalling; Approaches and Findi Lars Kaestner Book 2013 Springer-Verlag Berlin Heidelberg 2013 calcium signalling.cardiac myocytes

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Neue Wege zur Wassersterilisierung (Katadyn)odulation in calcium. This concept is a very complex approach because function can be modulated by many parameters that are directly or indirectly influenced by calcium. Therefore, primary and secondary effects are difficult or impossible to discriminate.

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Direct evidence - the digital approachter and distilled water and identified the presence of calcium as a necessary requirement for ECC. This early experiments hinted that the physiologically relevant calcium concentration is rather small and is in the µM range.

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Molecular biology based approachesed in other sources. Examples include high-pressure liquid chromatography (HPLC), 2D-gel electrophoresis, mass spectrometry, matrix-assisted laser desorption/ionisation in combination with time-of-flight mass spectrometer (MALDI-TOF) and expression analysis with DNA chips or next-generation sequencing.

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Manipulation of calciumn chapter 2 - Direct evidence - the digital approach. An even more elegant approach is to induce a transient disturbance in cardiomyocytes, the application of 20 mM caffeine leads to the opening of the internal calcium stores, namely the sarcoplasmic reticulum (SR), by opening the ryanodine receptors.

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Book 2013 calcium in living cells. The focus within these methods in on the broad range of fluorescence imaging technology, in particular on optical sectioning techniques and fast image acquisition. In addition to these general guidelines there are application examples in a context beyond calcium signalling

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