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Titlebook: Calcium signalling; Approaches and Findi Lars Kaestner Book 2013 Springer-Verlag Berlin Heidelberg 2013 calcium signalling.cardiac myocytes

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A primary culture system for sustained expression of a calcium sensor in preserved adult rat ventric medium supplemented with an insulin–transferrin–selenite mixture on substrates coated with extracellular matrix proteins showed an increased cell attachment and a conserved cross-striation. Moreover, these myocytes displayed optimised preservation of their contractile behaviour and Ca. signalling e
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https://doi.org/10.1007/978-3-322-85987-7 medium supplemented with an insulin–transferrin–selenite mixture on substrates coated with extracellular matrix proteins showed an increased cell attachment and a conserved cross-striation. Moreover, these myocytes displayed optimised preservation of their contractile behaviour and Ca. signalling e
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https://doi.org/10.1007/978-3-322-85987-7lation by prostaglandin E. and lysophosphatidic acid. For the latter stimulus, we recorded emission spectra of individual erythrocytes to confirm largely unaltered Fluo-4 emission. Our results emphasise that in erythrocytes measurements of intracellular calcium are reliably possible with Fluo-4 and
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Introductionminute amounts of calcium were necessary to maintain heart muscle contractility. Currently, approximately 130 years later, our knowledge of calcium as a versatile signalling molecule has increased tremendously, and we have a good understanding of its general principles. As described in these reviews
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