Titlebook: Borrelia burgdorferi; Methods and Protocol Utpal Pal,Ozlem Buyuktanir Book 2018 Springer Science+Business Media LLC 2018 bacterial pathogen

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https://doi.org/10.1007/978-3-658-23805-6of . proteome and protein–protein interactions, which play central roles in biological processes in diverse organisms, are critical in understanding biology and infectivity of spirochetes. Here, we describe the proteomic analysis of . by two-dimensional (2-D) gel electrophoresis followed by protein

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Co-immunoprecipitation for Identifying Protein-Protein Interactions in ,,co-immunoprecipitation protocol that can be used to examine protein complexes found in the pathogenic spirochete .. The method outlined here has successfully identified known and unknown members of borrelial protein complexes and is an efficient method for studying protein interactions in this pathogenic spirochete.

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1064-3745 thoritative and cutting-edge, .Borrelia burgdorferi:Methods and Protocols .aims to ensure successful results in the further study of this vital field of biomedical research..978-1-4939-8468-8978-1-4939-7383-5Series ISSN 1064-3745 Series E-ISSN 1940-6029

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Species Identification and Phylogenetic Analysis of , Sensu Lato Using Molecular Biological Methodsecies complex, the importance of accurate species and strain typing has come to the fore. This is particularly significant with a view of differentiating human pathogenic and non-pathogenic strains or species and understanding the epidemiology, ecology, population structure, and evolution of species

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Analysis of DNA and RNA Binding Properties of , Regulatory Proteins,escribed. The methods described are intended to facilitate empirical assessment of the binding affinity, co-factor requirements, quality of the interacting partners, and readily modifiable assay conditions to assess the binding properties to define known and unknown regulatory properties of nucleic

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Genome-Wide Mutagenesis in ,,rent time points using Luminex Flex.Map™ technology provides a robust data set. . transposon mutant screening indicates that a high proportion of genes are the novel virulence determinants that are required for mouse and tick infection. In this protocol, an effective signature-tagged .-based transpo