Titlebook: Borrelia burgdorferi; Methods and Protocol Utpal Pal,Ozlem Buyuktanir Book 2018 Springer Science+Business Media LLC 2018 bacterial pathogen

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1064-3745 ation advice from the experts.This volume details protocols that broadly cover many aspects of basic and translational research on .Borrelia burgdorferi. .Chapters guide readers through epidemiology and ecology, cultivation, cell structure, physiology, genomics and transcriptomics, proteomics, anima

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Schluss: Zusammenfassung und Ausblick,l caveats to consider. This chapter describes the design of basic molecular genetic experiments as well as the detailed methodologies to prepare and transform competent cells, select for and isolate transformants, and complement or genetically restore mutants.

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Analysis of Lipids and Lipid Rafts in ,,rafts define the ability of cells to form microdomains and compartmentalize the membrane. In this chapter, we describe the biophysical, biochemical, and molecular approaches used to define and characterize lipid rafts in the Lyme disease agent, ..

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Trace Element Analysis of , by Inductively Coupled Plasma-Sector Field Mass Spectrometry,onutrients and referred to as trace elements. Manganese (Mn), iron (Fe), copper (Cu), and zinc (Zn) are the most prevalent transition metals in the Lyme disease spirochete .. Here, we describe a method for the accurate measurement of these trace elements in . utilizing inductively coupled plasma-sector field mass spectrometry (ICP-SFMS).

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Genetic Transformation and Complementation,l caveats to consider. This chapter describes the design of basic molecular genetic experiments as well as the detailed methodologies to prepare and transform competent cells, select for and isolate transformants, and complement or genetically restore mutants.