Titlebook: Borrelia burgdorferi; Methods and Protocol Utpal Pal,Ozlem Buyuktanir Book 2018 Springer Science+Business Media LLC 2018 bacterial pathogen

IU421

书目名称Borrelia burgdorferi影响因子(影响力)




书目名称Borrelia burgdorferi影响因子(影响力)学科排名




书目名称Borrelia burgdorferi网络公开度




书目名称Borrelia burgdorferi网络公开度学科排名




书目名称Borrelia burgdorferi被引频次




书目名称Borrelia burgdorferi被引频次学科排名




书目名称Borrelia burgdorferi年度引用




书目名称Borrelia burgdorferi年度引用学科排名




书目名称Borrelia burgdorferi读者反馈




书目名称Borrelia burgdorferi读者反馈学科排名

真实的你

Martin Göbl,Andreas Froschmayerdetected Nε-lysine acetylation in diverse bacterial phyla, but no work on protein acetylation in . has been reported. Here, we describe a step-by-step protocol to identify Nε-lysine acetylated proteins in .

Transfusion

LUT

Genotyping Strains of Lyme Disease Agents Directly From Ticks, Blood, or Tissue,n the two loci allows for discrimination between strains representing all the areas of distribution. The methods presented here are meant for genotyping directly from ticks and from blood and tissue samples from vertebrates.

Etymology

Identification of Acetylated Proteins in ,,detected Nε-lysine acetylation in diverse bacterial phyla, but no work on protein acetylation in . has been reported. Here, we describe a step-by-step protocol to identify Nε-lysine acetylated proteins in .

意外的成功

,Analysis of , Proteome and Protein–Protein Interactions,identification via liquid chromatography-mass spectrometry and database searching. We also describe assays for studying the interaction between borrelial proteins: a novel high-throughput luciferase assay, yeast two-hybrid assay, and a far-Western assay that are routinely used in our laboratories.

indenture

Herbert Kopfer,Christian Bierwirtherived proteins interacting with complement regulator Factor H is described, including the preparation of whole cell lysates, the separation of proteins by Tris-Tricine SDS-PAGE, the transfer of the proteins to nitrocellulose membranes, and the detection of Factor H-interacting proteins by Far western.

圆桶

https://doi.org/10.1007/978-3-642-60184-2lates that are attenuated for infection in mice and thus cannot be naturally acquired by ticks. We also describe a quantitative PCR-based protocol for the measurement of . in tick and murine hosts targeting spirochete RNA that is highly efficient, reproducible, and a better surrogate of active infection.

BARB

intrude

Artificial Infection of Ticks with , Using a Microinjection Method and Their Detection In Vivo Usinlates that are attenuated for infection in mice and thus cannot be naturally acquired by ticks. We also describe a quantitative PCR-based protocol for the measurement of . in tick and murine hosts targeting spirochete RNA that is highly efficient, reproducible, and a better surrogate of active infection.