Titlebook: Borrelia burgdorferi; Methods and Protocol Utpal Pal,Ozlem Buyuktanir Book 2018 Springer Science+Business Media LLC 2018 bacterial pathogen

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Herbert Kopfer,Christian Bierwirth, inhibiting activation of complement as innate immunity’s first line of defense. Hence, the identification and characterization of interacting ligands is a prerequisite to gain deeper insights into the molecular principles of how spirochetes overcome the detrimental effects of complement. Far weste

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Martin Göbl,Andreas Froschmayerions of interest. Here, we describe modifications to employ this genome-wide screening method for ., the Lyme disease spirochete, during an active murine infection. Utilization of this technique provides valuable insights into the . transcriptome during infection, despite the low bacterial numbers i

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Martin Göbl,Andreas Froschmayerbind DNA or RNA in a given prokaryote. However, biological relevance of these regulatory proteins requires methods to rapidly purify and determine their binding properties within the physiological context or life style of the organism. Here, we describe the experimental approaches to determine the n

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Martin Göbl,Andreas Froschmayerne acetylation—the acetylation of the epsilon amino group of lysine residues. Nε-lysine acetylation is known to regulate multiple cellular processes. In eukaryotes, it regulates chromatin structure, transcription, metabolism, signal transduction, and the cytoskeleton. Recently, multiple groups have

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Schluss: Zusammenfassung und Ausblick,tants in these recalcitrant bacteria, while not straightforward, is routinely accomplished in numerous laboratories, although there are several crucial caveats to consider. This chapter describes the design of basic molecular genetic experiments as well as the detailed methodologies to prepare and t

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https://doi.org/10.1007/978-3-322-92074-4erate deletion strains lacking the gene of interest. To circumvent this limitation, it is possible to generate conditional mutants in which a copy of the gene of interest is introduced into the bacteria to compensate for the loss of the native allele. Expression of the non-native copy of the target

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