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Titlebook: Bone Research Protocols; Miep H. Helfrich,Stuart H. Ralston Book 20031st edition Humana Press 2003

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Human Osteoblast Cultureequent mineralization. Once a phase of active bone formation is completed the osteoblasts do not become senescent but instead redifferentiate into one of two other cell types: osteocytes and bone lining cells, both of which play a major role in the regulation of calcium homeostasis and bone remodeli
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Mineralizing Fibroblast-Colony-Forming Assaysrix. These cells interact with each other and with the extracellular matrix, and when cell populations are removed from the network they cease to function normally. In the past, bone cell differentiation was studied using histological methods in either whole embryos or organ cultures. Although this
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Isolated Osteoclast Cultures the cells responsible for resorbing bone. Osteoclasts are usually few in number relative to other cell types in bone and are difficult to isolate because they are contained in a hard tissue; in addition, they are at the end of their proliferation and differentiation cycle, presenting problems for t
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Primary Isolation and Culture of Chicken Osteoclastses: bone-resorbing osteoclasts (OCs) which are uniquely capable of dissolving and removing a small volume of bone, and bone-forming osteoblasts that subsequently fill in these lacunae or pits with new bone tissue. Whereas osteoblasts originate from mesenchymal cell precursors, OCs derive from hemato
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