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Titlebook: Bone Research Protocols; Miep H. Helfrich,Stuart H. Ralston Book 20031st edition Humana Press 2003

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发表于 2025-3-21 19:22:55 | 显示全部楼层 |阅读模式
期刊全称Bone Research Protocols
影响因子2023Miep H. Helfrich,Stuart H. Ralston
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学科分类Methods in Molecular Medicine
图书封面Titlebook: Bone Research Protocols;  Miep H. Helfrich,Stuart H. Ralston Book 20031st edition Humana Press 2003
影响因子The last decade has seen a tremendous advance in our understanding of bone biology. The genes responsible for the majority of rare inherited bone disorders have been identified and much progress has been made in the identification of genes in polygenic disorders such as Paget’s disease and complex multigene diseases such as osteoporosis. Transgenic technology has identified further genes, sometimes unexpectedly, with profound effects on bone. This wealth of new genetic information will undoubtedly lead to extensive cell biological studies to understand the mechanisms by which these gene products affect bone mass and bone strength. In Bone Research Protocols a catalogue of protocols has been assembled to perform such mechanistic studies. In the tradition of the Methods in Molecular Medicine series, the chapters are practical laboratory protocols that should enable the reader to carry out the techniques from scratch. To our knowledge this is the first time such a truly practical manual on well-established bone methods has been assembled, and this volume aims to be complementary to and follow on from the more theoretical Methods in Bone Biology, edited by Arnett and Henderson (1).
Pindex Book 20031st edition
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https://doi.org/10.1007/978-3-642-58641-5es, and antigenic profiles (.–.). Most importantly, they develop a capacity for bone pit resorption, the unique and defining functional attribute of OCs. Bone resorption and formation are normally carefully balanced processes in adults. However, in various diseases or pathological conditions, an imb
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J. Pettavel,S. Leyvraz,P. Douglas mature OCs exist and the few pre-OC cell lines that have been reported either do not undergo full OC differentiation (.,.) or involve coculture systems and cells that may not be readily available to all researchers (.–.). To compound the problem further, it has proven difficult or impossible until
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Cornelis J. H. Velde,Paul H. Sugarbakernd human .. More recently, techniques have been developed to generate osteoclasts in vitro (.,.) and then incubate these on bone slices to produce bone resorption. With these approaches, osteoclasts are defined by their ability to produce resorption pits on bone slices. However, this definition may
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Mineralizing Fibroblast-Colony-Forming Assaysxogenous agents in a similar manner to that observed in vivo (.). Recently a number of in vitro models have been established that re-create discrete elements of the cellular network present in the bone micro-environment. The advantage of these models is that they have reduced complexity compared wit
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Primary Isolation and Culture of Chicken Osteoclastses, and antigenic profiles (.–.). Most importantly, they develop a capacity for bone pit resorption, the unique and defining functional attribute of OCs. Bone resorption and formation are normally carefully balanced processes in adults. However, in various diseases or pathological conditions, an imb
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Osteoclast Formation in the Mouse Coculture Assay interactions. It has been widely used to study the origin of the osteoclast (.) and the effects of growth factors and drugs on osteoclast formation (.,.). In studies with osteopetrotic mice, the coculture assay has been used to determine whether the underlying mechanism was due to a defect in the o
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