Titlebook: Bone Research Protocols; Miep H. Helfrich,Stuart H. Ralston Book 20031st edition Humana Press 2003

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RANKL-Mediated Osteoclast Formation from Murine RAW 264.7 Cells mature OCs exist and the few pre-OC cell lines that have been reported either do not undergo full OC differentiation (.,.) or involve coculture systems and cells that may not be readily available to all researchers (.–.). To compound the problem further, it has proven difficult or impossible until

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熟练

使入迷

pantomime

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袋鼠

Assessing Bone Formation Using Mouse Calvarial Organ Culturesrtisone (.) and bisphosphonates (.). Others have used rodent calvarial and long bone explants to study the effects of cytokines on bone resorption (.–.) and bone formation (.,.). This chapter focuses on the use of cultured neonatal calvariae as an assay for agents with anabolic activity (.–.) (..).

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Shahid M. Hussain,Michael F. Sorrellines appear unstable to some extent. In addition, their clonal selection has favored rapidly growing cells, but has not necessarily selected for the whole range of bone-specific gene expression characteristic of primary bone cells. This means that in certain experiments, the use of primary bone cells is preferred to the use of cell lines.

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https://doi.org/10.1007/978-3-642-58641-5tes were outside the mainstream of bone research. Increasing interest in the mechanoregulation of bone has changed this, and today there is a general consensus that osteocytes play a pivotal role as mechanosensors and effectors in bone (.). Whether osteocytes have other functions remains to be elucidated.

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Bruno F. Fernandes,Miguel N. Burnier Jrrtisone (.) and bisphosphonates (.). Others have used rodent calvarial and long bone explants to study the effects of cytokines on bone resorption (.–.) and bone formation (.,.). This chapter focuses on the use of cultured neonatal calvariae as an assay for agents with anabolic activity (.–.) (..).