Titlebook: Artificial Riboswitches; Methods and Protocol Atsushi Ogawa Book 2014 Springer Science+Business Media New York 2014 RNA aptamers.artificial

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Fictional Worlds and the Moral Imaginationibraries are constructed by randomizing the nucleotides that potentially comprise an expression platform and fused to the hybrid selection/screening marker .–.. Iterative ON and OFF selections are performed under appropriate conditions that favor the survival or the growth of the cells harboring the

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https://doi.org/10.1007/978-3-031-52026-6nthetic mRNA switches that modulate translation of a target gene of interest in response to an intracellular protein could be a key regulator to construct a genetic circuit. Insertion of a protein binding RNA sequence in the 5′ UTR of mRNA would allow for the generation of a protein-responsive RNA s

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Culture, Recognition, and Poetry, for the control of gene expression in vivo, called artificial riboswitches. In this chapter, we describe how in vitro selection can be used to create an allosteric ribozyme that senses bacterial second messenger cyclic-di-GMP (c-di-GMP). A hammerhead ribozyme was joined to a natural c-di-GMP class

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,‘Keynotes’ of Decadence, 1894–95,ors and diagnostic devices will become more important in the future to allow for noninvasive determination of protein, ion, or small molecule metabolite levels. In recent years synthetic RNA-based switches have been developed to act as signal transducers to convert a binding event of a small molecul

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https://doi.org/10.1057/9780230504004c health problem. Enzymatic activation of antibiotics though the production of β-lactamase is one of the main mechanisms causing AR. Synthetic riboswitch containing aptazyme is constructed in . to regulate the expression of β-lactamase through small molecule–aptamer interactions, which sharply reduc