Titlebook: Artificial Riboswitches; Methods and Protocol Atsushi Ogawa Book 2014 Springer Science+Business Media New York 2014 RNA aptamers.artificial

团结

Engineered Riboswitch as a Gene-Regulatory Platform for Reducing Antibiotic Resistance,tch containing aptazyme is constructed in . to regulate the expression of β-lactamase through small molecule–aptamer interactions, which sharply reduces the antibiotic resistance of the engineered bacteria.

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Identification of RNA Aptamers Against Recombinant Proteins with a Hexa-Histidine Tag,rs against hexa-histidine-tagged proteins. For the efficient enrichment of higher affinity aptamers, the selection stringency should be gradually increased. Undesired RNA species that bind to affinity resins can be eliminated from the pool by using a negative selection step and alternating different types of resins.

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FUME

Guanine-Tethered Antisense Oligonucleotides as Synthetic Riboregulators,y of using G-ASs to modulate RNA conformation may allow control of RNA function by inducing biologically important quadruplex structures. This approach to manipulate quadruplex structures using G-ASs may expand the strategies for regulating RNA structures and the functions of short oligonucleotide riboregulators.

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Book 2014cules.. Artificial Riboswitches: Methods and Protocols. focuses on the state-of-the-art methods developed in recent years for creating artificial riboswitches, therefore this volume could be regarded as a collection of recipes for the gene circuit elements in synthetic biology and metabolic engineer

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Charlie Kuppens,Catherine M. Robbhich is easily available for the design of other ligand-dependent riboswitches, by introducing a new region (a modulator sequence: MS) in addition to the two basic regions. A facile method for preparing arbitrary molecule-dependent riboswitches based on the foundational riboswitch is also presented.

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,Development of Photoswitchable RNA Aptamer–Ligand Complexes,moiety that undergoes photoisomerization through light irradiation. Furthermore, we explain a procedure for surface plasmon resonance assay to detect photoswitchable association and dissociation of RNA–ligand complex on gold surface.