Titlebook: Artificial Riboswitches; Methods and Protocol Atsushi Ogawa Book 2014 Springer Science+Business Media New York 2014 RNA aptamers.artificial

冥想后

Rational Design of Artificial ON-Riboswitches,hich is easily available for the design of other ligand-dependent riboswitches, by introducing a new region (a modulator sequence: MS) in addition to the two basic regions. A facile method for preparing arbitrary molecule-dependent riboswitches based on the foundational riboswitch is also presented.

百灵鸟

Construction of Ligand-Responsive MicroRNAs that Operate Through Inhibition of Drosha Processing,, the engineered microRNAs can be readily modified to target different sequences and to bind different ligands. Individual miRNAs also can be incorporated into the same transcript for tunable, multi-gene silencing.

种子

Plato’s , as an Ethical Performancers against hexa-histidine-tagged proteins. For the efficient enrichment of higher affinity aptamers, the selection stringency should be gradually increased. Undesired RNA species that bind to affinity resins can be eliminated from the pool by using a negative selection step and alternating different types of resins.

阴谋小团体

Vital-Signs

Robust

Charlie Kuppens,Catherine M. Robby of using G-ASs to modulate RNA conformation may allow control of RNA function by inducing biologically important quadruplex structures. This approach to manipulate quadruplex structures using G-ASs may expand the strategies for regulating RNA structures and the functions of short oligonucleotide riboregulators.

callous

Extrahuman Transcendence in Oscar Wilde’s quence with potential binding affinity from a pool of random sequences and designed a protocol for RNA 3D structure prediction. The proposed approach significantly reduces the RNA sequence search space, thus accelerating the experimental screening and selection of high-affinity aptamers.

农学

腐烂

magnanimity

Dual-Selection for Evolution of In Vivo Functional Aptazymes as Riboswitch Parts,phenylalanine and a hammerhead ribozyme. Because this method uses selection, it does not rely on sequence-specific design and thus should be generalizable for the generation of in vivo operational aptazymes that respond to any targeted molecules.