期刊全称 | Artificial Riboswitches | 期刊简称 | Methods and Protocol | 影响因子2023 | Atsushi Ogawa | 视频video | | 发行地址 | Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts.Includes supplementary materia | 学科分类 | Methods in Molecular Biology | 图书封面 |  | 影响因子 | .Artificial riboswitches and other ligand-responsive gene regulators make it possible to switch protein synthesis ON or OFF with arbitrary ligand molecules.. Artificial Riboswitches: Methods and Protocols. focuses on the state-of-the-art methods developed in recent years for creating artificial riboswitches, therefore this volume could be regarded as a collection of recipes for the gene circuit elements in synthetic biology and metabolic engineering. Chapters cover topics such as screening or rational design methods for obtaining artificial riboswitches that function in either bacterial or eukaryotic translational systems, protocols for evaluating the activities of the resultant riboswitches, as well as protocols for construction of ligand-dependent, trans-acting gene regulators. Written in the successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls..Authoritative and easily accessible, .Artificial Riboswitches: Methods and Protocols. seeks to serve not only bioengineers who | Pindex | Book 2014 |
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