Titlebook: Artificial Nucleases; Marina A. Zenkova Book 2004 Springer-Verlag Berlin Heidelberg 2004 Catalysis.Cleavage.Conjugates.DNA.Enzyme mimics.N

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Günther Schlag,Peter Bösch,Helene Matraser natural ribonucleases such as: higher RNA sequence cleavage specificity, sequence flexibility in designing the site of RNA cleavage, and a wider range of conditions at which artificial ribonucleases are functional (Komiyama et al. 2001).

CONE

P. G. Friedl,G. Schürmann,Ch. Herfarthtions in biotechnology, for manipulating RNA, and in drug design. A direct approach to design of artificial RNases consists in imitation of the active centers of natural enzymes by simple organic structures possessing minimal sets of the most important characteristics that are essential for catalysi

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https://doi.org/10.1007/978-3-642-85101-8to behave as salts in solution since the negative charges of the polynucleotide phosphodiester backbone are usually neutralized by metal solute ions. Therefore, for practical purposes, it is difficult if not impossible to separate the behavior of DNA and RNA from their interactions with metal ions.

BILK

phase-2-enzyme

G. Spilker,R. Türk,A. Stemberger,G. Blümelcid is reflected in a number of reviews (Strater et al. 1996; Wilcox 1996; Cowan 1998; Kovall and Matthews 1999). In enzymes, interactions between functional groups of amino acid side radicals result in complex tertiary and quaternary structures that determine the composition of different functional

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https://doi.org/10.1007/978-3-642-69655-8f magnesium ion predominates in natural nucleolytic enzymes owing to high abundance of these ions, redox inertness, ligand exchange rates and charge density. However, other metal ions and multinuclear metal centers have also been identified in phosphate ester hydrolase enzymes with Zn.,Fe. and Ca. (

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Milzklebung im Kleintierversuch ohne Vliesonal groups of amino acid residues from catalytic centers of natural RNases: conjugates containing imidazole and carboxylate ions, guanidinium groups (see review Silnikov and Vlassov 200l). This chapter is devoted to proteins displaying ribonuclease activity and peptide-based conjugates which are ca

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B. Kessler,M. Arndt,R. E. Zimmermann If only one RNA can be chosen from many other RNAs and selectively cleaved at the desired site, it opens the way to new RNA science (regulation of expression of a specific gene in cells, advanced therapy, RNA manipulation, and others). However, none of the naturally occurring ribonucleases shows su

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Milzklebung im Kleintierversuch ohne Vlies998; Sil’nikov and Vlassov 2001). Apart from being useful molecular biology tools (Hüber 1993; Giegé et aL 2000), such compounds may provide new opportunities for design of therapeutics targeting specific messenger RNAs and viral genomic RNAs (Uhlmann and Peymann 1990; Komyiama and Sumaoka 1998; Tra

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