Titlebook: Arabidopsis Protocols; José M. Martinez-Zapater,Julio Salinas Book 19981st edition Humana Press 1998

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Postulate

Callus Culture and RegenerationAlternatively, plants can be regenerated from unorganized callus tissues derived from different explants by dedifferentiation induced by exogenous growth regulators. Plant regeneration from call1 is possible by . organogenesis or somatic embryogenesis. Callus cultures also facilitate the amphficatio

诱使

Schlemms-Canal

Goblet-Cells

Purification of Mitochondria from Arabidopsisdes most of the nonphotosynthetrc energy required m the cell. Mitochondria contain their own genome(s), but encode only a small number of vita1 gene products. Simrlar plastids, the majority of the organellar proteins are nuclear encoded, and imported into these organelles.

patella

Preparation of DNA from , isolation are often designed to produce large amounts of DNA of high molecular weight with sufficient purity. Since the polymerase chain reaction (PCR) 1s the method of choice in modern plant genetic analyses such as map-based breeding and positional gene clonmg, many recently developed protocols a

jettison

micronized

Chloroplast DNA Isolationatory procedure for isolating chloroplast DNA from . with minimal nuclear or mitochondrial DNA contamination. In general, a first step in isolating chloroplast DNA is the homogenization of the plant material followed by a filtration step to remove large-sized cell debris and cell fragments. The filt

Rustproof

12 Purification of Mitochondrial DNA from Green Tissues of Arabidopsis DNA (mtDNA). A prerequisite to purify mtDNA with little contamination of nuclear and also of plastid DNA is the isolatton of mitochondrta from cells. Mitochondria from green plants are generally dtfticult to purify because of the low amount of these organelles and the high number of chloroplasts m