Titlebook: Arabidopsis Protocols; José M. Martinez-Zapater,Julio Salinas Book 19981st edition Humana Press 1998

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Ausgangslage und theoretische Grundlagen,andbook of genetics. It is only owing to the specific (reproductive) biology of a species that sometimes a certain analytical procedure is favored over another one For . the fact that it is a self-fertilizing species and that it has relatively small flowers are important in this respect.

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Book 19981st editionin every area of plant biology, have served to mobilize resources and orient much new research towards the plant field, where the special biological features of Arabidopsis have further focused attention on this useful species. The high level of interest in Arabidopsis has worked as a catalyst and,

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Sterile Techniques in Arabidopsisnd catabolism of the compound by other organisms. Aside from the ease of screening, Petri plate screens also have the advantage that retesting and genetic analysts of subsequent generations can be done under exactly the same condmons as the original screen.

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Chloroplast DNA Isolationhloroplast DNA because intact chloroplasts have a nonporous envelope. In the second method, the intact chloroplasts are banded in a sucrose— or a percoll—density—gradient, while the nuclei pellet. Banded chloroplasts are carefully removed. Intact chloroplasts, obtained by either a DNase method or a

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12 Purification of Mitochondrial DNA from Green Tissues of Arabidopsisltures are available. Furthermore, tissue cultures are not always the ideal source for mvestigations of mitochondrial genome structure, since frequent reorganizattons have been reported for such tissue cultures, which distort the inplanta structure of these genomes (.,.).

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Preparation of RNAalso spawned variations, most notably a single‐step method that ehmmates an ultracentrifugation step (3). Although this method may compare favorably with the procedures presented herein with regard to technical simplicity, our protocol consistently results m high yields of intact RNA of a quality su

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