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Titlebook: Arabidopsis Protocols; José M. Martinez-Zapater,Julio Salinas Book 19981st edition Humana Press 1998

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期刊全称Arabidopsis Protocols
影响因子2023José M. Martinez-Zapater,Julio Salinas
视频videohttp://file.papertrans.cn/161/160592/160592.mp4
学科分类Methods in Molecular Biology
图书封面Titlebook: Arabidopsis Protocols;  José M. Martinez-Zapater,Julio Salinas Book 19981st edition Humana Press 1998
影响因子The use of Arabidopsis in genetics and biology laboratories can be traced back to the beginning of the century, when the small size of this plant species, its short generation time, and the thousands of seeds produced by each plant attracted the interest of geneticists. In the middle of the century, this plant had already gained a place in several genetics laboratories both in Europe and the United States, being widely used in mutagenesis experiments. However, exponential growth in the use of Arabidopsis as a model system did not get started until the 1980s, when the small size of its genome was realized, thus triggering the interest of geneticists and molecular biologists, not only from the plant field, but from other research fields needing a novel model system. In recent years, high expectations for Plant Biotechnology in the next century, together with the need for basic information in every area of plant biology, have served to mobilize resources and orient much new research towards the plant field, where the special biological features of Arabidopsis have further focused attention on this useful species. The high level of interest in Arabidopsis has worked as a catalyst and,
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Methods in Molecular Biologyhttp://image.papertrans.cn/b/image/160592.jpg
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Erziehungsziel "Selbstständigkeit"ia that can be employed include soil, commercial greenhouse mixes, vermrcuhte, and other relatively inert media watered wtth nutrient solutions, as well as agar. This chapter focuses on growth of plants on so11 in various envrromnental settings and especrally m growth chambers and greenhouses. Harve
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Erziehungsziel "Selbstständigkeit"are mamtamed for mapping analysis, and potentially useful new mutants are not lost m the early fragile stages of screening. . 1s prone to many of the pests that commonly infect greenhouses and growthrooms (.,.). These can cause severe damage to the plants and, m some cases, can kill them. In general
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Konzepte konstruktivistischen Denkensed (.). Cell suspensions are used for generating large amounts of cells for quantitative or qualitative analysis of growth responses and metabolism of novel chemicals, as well as for studies of cell cycle under standard conditions (.,.). In addition, cell suspensions serve as an ideal material for t
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Erziehungsziel "Selbstständigkeit"Alternatively, plants can be regenerated from unorganized callus tissues derived from different explants by dedifferentiation induced by exogenous growth regulators. Plant regeneration from call1 is possible by . organogenesis or somatic embryogenesis. Callus cultures also facilitate the amphficatio
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Erziehungsziel "Selbstständigkeit" problems in achieving a high frequency of sustainable division of protoplasts in liquid culture. The problem can be alleviated to a certain extent by embedding mesophyll protoplasts m calcium algmate (.). We have developed a technique allowing the culture of protoplasts derived from roots or cell s
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