Titlebook: Arabidopsis Protocols; José M. Martinez-Zapater,Julio Salinas Book 19981st edition Humana Press 1998

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Problemstellung und Vorgehensweise isolation are often designed to produce large amounts of DNA of high molecular weight with sufficient purity. Since the polymerase chain reaction (PCR) 1s the method of choice in modern plant genetic analyses such as map-based breeding and positional gene clonmg, many recently developed protocols a

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Erziehungsziel "Selbstständigkeit"iving tissues generally do not allow recovery of DNA fragments larger than 50‐100 kb m size. In recent years, technical improvements have made possible to purify and analyze large DNA molecules in vitro. Techniques such as pulsed‐field agarose gel electrophoresis (PFGE) (.) or cloning m yeast artifi

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Problemstellung und Vorgehensweise DNA (mtDNA). A prerequisite to purify mtDNA with little contamination of nuclear and also of plastid DNA is the isolatton of mitochondrta from cells. Mitochondria from green plants are generally dtfticult to purify because of the low amount of these organelles and the high number of chloroplasts m

乐器演奏者

Konzepte konstruktivistischen Denkensure that bridges the interface between the experimental manipulation of the hvmg system, and the subsequent analysis of effects through molecular techniques. . is an ideal system for such analyses. Large quantities of plant material are easily obtained, from which mrlligram quantities of total RNA m

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Ausgangslage und theoretische Grundlagen,andbook of genetics. It is only owing to the specific (reproductive) biology of a species that sometimes a certain analytical procedure is favored over another one For . the fact that it is a self-fertilizing species and that it has relatively small flowers are important in this respect.

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Arabidopsis Protocols978-1-59259-268-5Series ISSN 1064-3745 Series E-ISSN 1940-6029