Titlebook: Antiviral Resistance in Plants; Methods and Protocol John M. Watson,Ming-Bo Wang Book 2012 Springer Science+Business Media, LLC 2012 Antivi

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Use of Hairpin RNA Constructs for Engineering Plant Virus Resistance,ng that leads to high-level virus resistance is triggered by virus-specific double-stranded (ds)RNA. The most efficient means to produce such dsRNA in transgenic plants is the introduction and expression of hairpin (hp) RNA constructs. Successful induction of the RNA silencing pathway is witnessed b

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Virus-Induced Gene Silencing in Soybean,d genomic tools are available for studies in the crop plant soybean, investigations related to gene function have been lacking. This is largely due to the fact that rapid functional genomics tools have been unavailable for application in soybean. This constraint was recently alleviated when a novel

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Book 2012ed to the vital discovery of double-stranded RNA (dsRNA)-induced gene silencing or RNA interference (RNAi), which has since revolutionized research on plant-virus interactions. In .Antiviral Resistance in Plants: Methods and Protocols., expert researchers in the field detail many of the methods whic

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Long Wang,Justus Bross,Christoph Meinelan agroinfiltration assay and grafting experiments, which were used for the characterization of . 50 kDa movement protein as a gene silencing suppressor. This protocol should allow the investigator to characterize putative plant virus-encoded gene silencing suppressors.