Titlebook: Antiviral Resistance in Plants; Methods and Protocol John M. Watson,Ming-Bo Wang Book 2012 Springer Science+Business Media, LLC 2012 Antivi

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Hans J. Scholl,Eric E. Holdemanthe mild virus strain to establish MSCP. To investigate interference among virus strains and the plant host at an early stage of MSCP at a subcellular level, we developed a rapid micro-extraction method for the preparation of total nucleic acid (TNA), combined with other molecular methods, to monito

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Towards Interoperable Open Statistical Datared approximately 10 years later, and much effort has subsequently been devoted to simplifying the sample preparation procedure and identifying group-specific primer pairs. This chapter describes four simple, easy-to-follow protocols—two involving PAGE and two others based on enzymatic amplification

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Creating Local Government Innovatione assignment of functions to specific genes. The strategy involves inserting short sequences of target soybean genes into the viral genome to generate recombinant vectors. Soybean plants are then inoculated with the recombinant vectors, assessed for BPMV propagation and silencing of the target genes

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Alexander van Deursen,Jan van DijkThe goal of this chapter is to describe in simple terms how the use of ordinary differential equation (ODE) modeling, in conjunction with experimentation, can be utilized to improve our understanding of the dynamics of gene silencing and virus resistance in plants.

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