Titlebook: Antiviral Resistance in Plants; Methods and Protocol John M. Watson,Ming-Bo Wang Book 2012 Springer Science+Business Media, LLC 2012 Antivi

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RNA Silencing and Antiviral Defense in Plants,. Since the publication of landmark papers in 1998 (Fire et al., Nature 391:806–811, 1998; Waterhouse et al., Proc Natl Acad Sci U S A 95:13959–13964, 1998), intense research efforts have resulted in much progress from the speculation of Mello and colleagues that “the mechanisms underlying RNA inter

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Biological Chemistry of Virus-Encoded Suppressors of RNA Silencing: An Overview,e molecular immune mechanism against invading viruses. The antiviral RNAi pathway is initiated with the generation of virus-derived short-interfering RNAs (siRNAs) that are used for subsequent sequence-specific recognition and degradation of the cognate viral RNA molecules. As an efficient counter-d

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Generation of Plant Small RNA cDNA Libraries for High-Throughput Sequencing,in plants. Conventional cloning and sequencing approaches have identified hundreds of miRNAs and a large number of siRNAs, but are no longer the best choices for identification of new miRNAs which are generally expressed at low abundance. The development of next-generation sequencing technologies ha

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Isolation and Detection of Small RNAs from Plant Tissues,l processes. The two main classes of sRNA, the small-interfering RNA (siRNA) and microRNA (miRNA) classes, are well documented and several experimental approaches have been developed to allow for their routine isolation and detection from plant tissues. Here, we describe the current methods used for

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