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Titlebook: Animal Influenza Virus; Methods and Protocol Erica Spackman Book 2020Latest edition Springer Science+Business Media, LLC, part of Springer

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Agar Gel Immunodiffusion Assay to Detect Antibodies to Type A Influenza Virustrix (M) proteins. Therefore, this test will detect antibodies to all influenza A virus subtypes. AGID is commonly used to screen poultry flocks for avian influenza virus infection. The AGID is a simple and economical serological test. All serological testing has its advantages and disadvantages, wh
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Conducting Influenza Virus Pathogenesis Studies in Avian Specieshanisms of transmission, viral pathotype, and viral evolution. Complex systems and natural settings may also be explored with carefully designed infection studies. In this chapter, we explore the elements of infection studies, general guidelines for choosing a virus to use, host selection, and many
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Laboratory Methods for Assessing and Licensing Influenza Vaccines for Poultryn doses have been used between 2002 and 2018 for high pathogenicity AI control. No universal vaccines are currently available. The majority of AI vaccines are inactivated whole influenza viruses that are grown in embryonating chicken eggs, emulsified in oil adjuvant systems, and injected subcutaneou
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Agar Gel Immunodiffusion Assay to Detect Antibodies to Type A Influenza Virusich should be considered before choosing the optimal test for the laboratory needs. Each laboratory must evaluate the laboratory’s resources, the volume of testing, the goal of testing, how the test results are used, and what types of samples are being tested in order to select the optimal test.
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Ecological Modelling Applied to Entomologyaboratory temperature, laboratory equipment, technical expertise of the user) may contribute to slight differences in the interpretation of the test each time it is run. This chapter will describe the methods validated and used by the US National Veterinary Services Laboratories for screening and identification of hemagglutinating viruses.
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