Titlebook: Animal Cell Electroporation and Electrofusion Protocols; Jac A. Nickoloff Book 1995 Springer Science+Business Media New York 1995

Exposure

Electroporation of Human Lymphoblastoid Cellsoducts on DNA replication (.–.), repair (.–.), recombination (.), or gene expression (.–.) can be performed. Alternatively, vectors that specifically respond to a particular enzymatic function, such as homologous recombination (.,.) or mismatch repair (.), can be introduced to determine whether a ce

fulcrum

Incumbent

Electroporation of Embryonic Stem Cells for Generating Transgenic Mice and Studying In Vitro Differeot until about 10 years ago did the true potential for the genetic manipulation of cells become a reality, with the ability to alter a mammalian gene precisely by targeted homologous recombination (.–.). This powerful tool was first devised for gene therapy approaches, but was quickly adopted for th

精密

上釉彩

Effect of Cis-Located Human Satellite DNA on Electroporation Efficiency genes containing appropriate expression signals will, in most cases, be expressed both prior to and after integration. However, stable expression of the transfected genes is not always observed (.,.). One reason this is believed to occur is because the site of integration of the foreign DNA is virt

聪明

杀菌剂

Stable Integration of Vectors at High Copy Number for High-Level Expression in Animal Cellsl to the study and the therapeutic utility of a wide variety of proteins. . expression systems are useful for many proteins. However, large complex proteins are not expressed efficiently in bacteria. Also, bacterial expression cannot be used for eukaryotic proteins that require glycosylation for the

Lumbar-Spine

逗留

Gordian Schudt,Olga Dolnik,Stephan Beckerully under otherwise mild conditions; and to insert membrane macromolecules into the cell membrane itself. Only recently has the exploration of in vivo electroporation for use with intact tissue begun. Several possible applications have been identified, viz. combined electroporation and anticancer d

genesis