Titlebook: Animal Cell Electroporation and Electrofusion Protocols; Jac A. Nickoloff Book 1995 Springer Science+Business Media New York 1995

杀虫剂

Electroporation of Embryonic Stem Cells for Generating Transgenic Mice and Studying In Vitro Differee transfection (.) and genetic disruption of genes in embryonic stem (ES) cell lines to produce transgenic animals (.,.). Currently, the most common cell line used is the mouse ES cell, but ES cells have recently been derived for other species (rabbit, rat, and hamster) (.–.).

Conspiracy

Effect of Cis-Located Human Satellite DNA on Electroporation Efficiencyually random with respect to both the exogenous DNA and the particular human chromosome (.). Therefore, it can be expected that not only the expression, but also the stability and copy number of the transfected gene will depend on the integration sites and chromosomal flanking sequences.

Myelin

夸张

变异

魔鬼在游行

Hans Dieter Klenk,Werner Slenczka cells (.–.). Electroporation has the advantage over other techniques in that many cells can be treated simultaneously and one or more compounds or macromolecules, such as proteins, can be introduced into cells at the same time (..).

Accord

GULF

P. Flammang,R. Santos,D. Haesaertsually random with respect to both the exogenous DNA and the particular human chromosome (.). Therefore, it can be expected that not only the expression, but also the stability and copy number of the transfected gene will depend on the integration sites and chromosomal flanking sequences.

蒸发

P. Flammang,R. Santos,D. Haesaertsir activity. Some established animal cell lines can be used as production hosts since they are easily transfected, can grow to high cell densities, and can be propagated in low-serum or serum-free medium.

严重伤害