Titlebook: Animal Cell Electroporation and Electrofusion Protocols; Jac A. Nickoloff Book 1995 Springer Science+Business Media New York 1995

Nutraceutical

书目名称Animal Cell Electroporation and Electrofusion Protocols影响因子(影响力)




书目名称Animal Cell Electroporation and Electrofusion Protocols影响因子(影响力)学科排名




书目名称Animal Cell Electroporation and Electrofusion Protocols网络公开度




书目名称Animal Cell Electroporation and Electrofusion Protocols网络公开度学科排名




书目名称Animal Cell Electroporation and Electrofusion Protocols被引频次




书目名称Animal Cell Electroporation and Electrofusion Protocols被引频次学科排名




书目名称Animal Cell Electroporation and Electrofusion Protocols年度引用




书目名称Animal Cell Electroporation and Electrofusion Protocols年度引用学科排名




书目名称Animal Cell Electroporation and Electrofusion Protocols读者反馈




书目名称Animal Cell Electroporation and Electrofusion Protocols读者反馈学科排名

抛媚眼

Instrumentationuration, and wave form. In this chapter, the term electro cell manipulation (ECM) shall describe both techniques. ECM is a quite universal technique that can be applied to eggs, sperm, platelets, mammalian cells, plant protoplasts, plant pollen, liposomes, bacteria, fungi, and yeast—generally to any

heterogeneous

The Introduction of Proteins into Mammalian Cells by Electroporationtroporation, or after transfection of cells with expression vectors containing the gene(s) of interest. Unlike most of these other methods, electroporation is a simple, relatively inexpensive, and efficient method for rapidly introducing DNA, small molecules, and proteins into bacterial or mammalian

混合物

Electroporation of Antigen-Presenting Cells for T-Cell Recognition and Cytotoxic T-Lymphocyte Priminadicating tumor cells (.–.). CTL normally recognize endogenously processed peptide antigens (Ag) complexed to MHC class I molecules (.,.). Immunization with virus-infected antigen-presenting cells (APC) or Ag-expressing transfectants, and even APC osmotically loaded with antigens can elicit CD8. cyt

整体

椭圆

osculate

Electrotransformation of Chinese Hamster Ovary Cellsy are easy to culture, they grow rapidly without density dependence, and they do not have stringent culture medium or high serum growth requirements. CHO cell lines have a high plating efficiency, and many lines can grow either as a monolayer or in suspension. These features, and techniques for repl

SYN

Electroporation of Rat Pituitary Cellshis technique has also been used for important functional studies in the different GH cell strains: rat pituitary tumor cells that produce prolactin or growth hormone. In most cases, transient-expression experiments have been used to study cellular processes, such as rat growth hormone gene expressi

intuition

Electroporation of Plasmid DNA into Normal Human Fibroblastsoften been used because of their finite life-span. Instead, immortal human cell lines have usually been used as genetic recipients. These cell lines derived from tumors or by transformation with SV40 virus have accumulated numerous genetic changes (.,.), and may in fact contain changes that will aff

衣服

Electroporation-Mediated Gene Transfer into Hepatocytese been developed that utilize retroviruses (.), polycations (.), liposomes (.), chromosomes (.), reconstituted viral envelopes (.) and other chemical reagents, such as calcium phosphate (.), DEAE-dextran (.), and lipopolyamines (.). DNA also can be transferred into cells by physical means, such as m