Titlebook: Ancient DNA; Methods and Protocol Beth Shapiro,Axel Barlow,André E. R.‘Soares Book 2019Latest edition Springer Science+Business Media, LLC,

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Role of Drebrin in Synaptic Plasticityof ancient human remains are often complicated by contamination with present-day human DNA, which can be introduced during excavation and subsequent handling of the specimens. In most cases, the relative abundance of contaminant DNA is much greater than that of the target organism. Here we present t

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Role of Drebrin in Synaptic Plasticity between samples and substrates but usually constitutes only a small fraction of the total DNA, while the remainder comprises contamination deriving from bacteria, fungi, and other microorganisms and in some cases also modern human DNA. Recently, several protocols have been developed to improve acce

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https://doi.org/10.1007/978-4-431-56550-5ard tissue matrix is degraded using an EDTA/Proteinase K lysis buffer, and the DNA is purified using spin columns with silica membranes. This method efficiently recovers molecules as short as 35 base-pairs long.

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https://doi.org/10.1007/978-94-015-2902-0etics over Quaternary timescales using ancient DNA (aDNA)-based analyses. A variety of plant tissues, preserved primarily by desiccation and anaerobic waterlogging, have proven to be viable sources of aDNA. Plant tissues are anatomically and chemically diverse and therefore require optimized DNA ext

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https://doi.org/10.1007/978-94-015-2808-5 but such methods are still lacking for other sequencing platforms. Here, we provide a protocol for building genomic libraries from degraded DNA (archival or ancient sample material) for sequencing on the Ion Torrent™ high-throughput sequencing platforms. In addition to a reduction in time and cost