Titlebook: Ancient DNA; Methods and Protocol Beth Shapiro,Axel Barlow,André E. R.‘Soares Book 2019Latest edition Springer Science+Business Media, LLC,

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Book 2019Latest editionancient samples for DNA extraction, the DNA extraction itself, and transforming extracted ancient DNA molecules for sequencing library preparation. The volume also examines the analysis of high-throughput sequencing data recovered from ancient specimens, which, because of the degraded nature of anci

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Extraction of Ancient DNA from Plant Remains,raction approaches. Here, we describe a plant DNA isolation protocol that performs well in most contexts. We include recommendations for optimization to retain the very short DNA fragments that are expected to be preserved in degraded tissues.

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Sequencing Library Preparation from Degraded Samples for Non-illumina Sequencing Platforms,in comparison to commercial kits, this protocol removes purification steps prior to library amplification, an important consideration for work involving historical samples. Libraries prepared using this method are appropriate for either shotgun sequencing or enrichment-based downstream approaches.

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Book 2019Latest edition cutting-edge, .Ancient DNA: Methods and Protocols, Second Edition. aims to serve both experts and beginners by presenting protocols in a manner that makes them easily accessible for everyday use in the lab..

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https://doi.org/10.1007/978-94-017-7137-5tion of repetitive regions in the DNA library; and (3) performing in vitro transcription of the repetitive region-depleted DNA library to generate biotinylated RNA baits. Where applicable, we include notes and recommendations based on our own experiences.

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Vektorkugelfunktionen und Ihre Anwendungen,s (e.g., plants, mammals), using the polymerase chain reaction (PCR) with established generic “barcode” primers. We also provide a catalog of animal and plant barcode primers that, because they target relatively short fragments of DNA, are potentially suitable for use with degraded DNA.

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1064-3745 lls. .Authoritative and cutting-edge, .Ancient DNA: Methods and Protocols, Second Edition. aims to serve both experts and beginners by presenting protocols in a manner that makes them easily accessible for everyday use in the lab..978-1-4939-9176-1Series ISSN 1064-3745 Series E-ISSN 1940-6029