Titlebook: Ancient DNA; Methods and Protocol Beth Shapiro,Axel Barlow,André E. R.‘Soares Book 2019Latest edition Springer Science+Business Media, LLC,

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https://doi.org/10.1007/978-94-017-6236-6 are not suitable for aDNA or other types of damaged DNA. Here, we outline a protocol for HTS library preparation that is optimized for ancient DNA. We report the library conversion rate for a range of input template and adapter concentrations. Our results show that the protocol performs at a high efficiency.

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Sampling and Extraction of Ancient DNA from Sediments, extraction protocols may need to be optimized for the recovery of DNA from sediments, which may contain inhibitors. Here we describe procedures for subsampling both nonfrozen and frozen sediment cores, and we describe an efficient method for ancient DNA extraction from such samples.

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Hiroyuki Yamazaki,Tomoaki Shirao working with ancient samples, including providing guidelines for setting up a new ancient DNA laboratory. We also discuss steps that can be taken at the sample collection and preparation stage to minimize the potential for contamination of ancient DNA experiments with exogenous sources of DNA.

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Role of Drebrin in Synaptic Plasticityeach) pretreatment to destroy contaminant DNA that may be bound or otherwise attached to the surface of bone/tooth powder. The second, less destructive pretreatment uses a phosphate buffer to release surface-bound DNA.