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Titlebook: Affinity Chromatography; Methods and Protocol Michael Zachariou (Director Project Management) Book 2008Latest edition Humana Press 2008 Chr

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Amylose Affinity Chromatography of Maltose-Binding Protein bind or modify the matrix during purification. Chromatography conditions are presented for purification at very small scales of less than 0.5 mL using amylose magnetic beads, a batch and semi-batch method for small to moderate scale purifications up to approximately 35 mg and larger scale FPLC meth
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Book 2008Latest editionof affinity chromatography step, while a database search of PubMed reveals more than 36,000 publications making use of the term “affinity chromatography,” more than 3000 of which refer to it in their title. The US patent office reports more than 16,000 ref- ences to the term “affinity chromatography
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Gian-Claudio Gentile,Theo Wehnere absolute specificity of a biological ligand/ligate system such as the avidin/biotin interaction or an antibody and its cognate antigen, IMAC can sometimes display non-ideal product purity. The protocols described in this chapter provide strategies for the improvement in the purity of IMAC-purified
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