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Titlebook: Affinity Chromatography; Methods and Protocol Michael Zachariou (Director Project Management) Book 2008Latest edition Humana Press 2008 Chr

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期刊全称Affinity Chromatography
期刊简称Methods and Protocol
影响因子2023Michael Zachariou (Director Project Management)
视频video
发行地址Contains state-of-the-art, brand new protocols developed for this second edition.Provides step-by-step laboratory instructions for readily reproducible results
学科分类Methods in Molecular Biology
图书封面Titlebook: Affinity Chromatography; Methods and Protocol Michael Zachariou (Director Project Management) Book 2008Latest edition Humana Press 2008 Chr
影响因子Forty years after the term “affinity chromatography” was introduced, this mode of chromatography remains a key tool in the armory of separation techniques that are available to separation and interaction scientists. Affinity chromat- raphy is favored because of its high selectivity, speed, and ease of use. The rapid and selective isolation of molecules using affinity chromatography has allowed a better understanding of biological processes, accelerated the identi- cation of target molecules, and spawned new process areas such as immobilized enzyme reactors. It has had ubiquitous application in most areas of science ranging from small molecule isolation to biopolymers such as DNA, proteins, polysaccharides, and even whole cells. The number of applications of affinity chromatography continues to expand at a rapid rate. For example, more than 60% of purification protocols include some sort of affinity chromatography step, while a database search of PubMed reveals more than 36,000 publications making use of the term “affinity chromatography,” more than 3000 of which refer to it in their title. The US patent office reports more than 16,000 ref- ences to the term “affinity chromatography
Pindex Book 2008Latest edition
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Affinity Precipitation of Proteins Using Metal ChelatesThe copolymers of vinylimidazole with .-isopropylacrylamide are easily synthesized by radical polymerization. When loaded with Cu(II) and Ni(II) ions, these copolymers are capable of selectively precipitating proteins with natural metal-binding groups or histidine-tagged recombinant proteins.
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Dye Ligand Chromatographyanalogs, offering affinity interactions with their corresponding enzymes. This chapter describes a dye ligand chromatography technique for purifying proteins from overexpression, in mammalian cell culture. The method begins with batch binding in order to rapidly select binding and elution conditions
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Purification of Proteins Using Displacement Chromatographyurified components at high concentrations, less tailing during elution, high throughput and high resolution. Displacer affinity and its utilization are the critical components of displacement chromatography. Particularly, the nonspecific interactions between the displacer and the stationary phase ca
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Phage Display of Peptides in Ligand Selection for Use in Affinity Chromatographyghtforward process involving several cycles of selection against target molecules, and the resulting ligands can be tailored to various applications. In this chapter we describe detailed methods to select peptide ligands for affinity chromatography, with particular focus on selection of peptides tha
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Preparation, Analysis and Use of an Affinity Adsorbent for the Purification of GST Fusion Proteine (GST) fusion protein in packed and expanded bed chromatographic processes. The protein is composed of GST fused to a zinc finger transcription factor (ZnF). Glutathione, the affinity ligand for GST purification, is covalently immobilized to a solid-phase adsorbent (Streamline™). The GST–ZnF fusion
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Immobilized Metal Ion Affinity Chromatography of Histidine-Tagged Fusion Proteinspression vectors for the production of histidine-tagged recombinant proteins as well as the variety of stationary supports for their separation Make IMAC an attractive and versatile choice for fast and reliable protein purification. It is not uncommon for IMAC purification to yield near homogenous t
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