Titlebook: Affinity Chromatography; Methods and Protocol Michael Zachariou (Director Project Management) Book 2008Latest edition Humana Press 2008 Chr

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Methods for the Purification of HQ-Tagged Proteinsbe expressed and purified from bacterial cells under native and denaturing conditions, mammalian cells, insect cells, wheat germ and rabbit reticulocyte. Furthermore, HQ-tagged proteins can be purified using magnetic or non-magnetic resins in multiple formats from small to large-scale and manual or

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Site-Specific Cleavage of Fusion Proteins such fusion partners from the intended protein product by cleavage with site-specific endoproteases. Methods to achieve proteolytic cleavage of the fusion proteins are provided, along with techniques for optimizing the yield of authentic product.

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Phage Display of Peptides in Ligand Selection for Use in Affinity Chromatographythentic epitope mimic and coupling the peptide mimotope to an affinity resin for purifying antibodies from human serum. There are several other applications of phage peptides that could be used for affinity chromatography; the approaches are outlined, but detailed methods have not been included.

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The Basics of Financial Statement Analysise for purifying proteins from over-expression in mammalian cell culture. The immobilization of the monoclonal antibody or polyclonal antiserum is presented. Conditions for purifying up to milligram quantities of protein are given, including a representative chromatogram.

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An Overview of Corporate Valuationn be exploited to generate high affinity displacers. This chapter will discuss the design and execution of displacer selection and implementation in a separation specifically focusing on its utilization in ion exchange chromatography.