Overview: .This book constitutes the thoroughly refereed proceedings of the 6th International Workshop on Hybrid Systems Biology, HSB 2019, held in Prague, Czech Republic, in April 2019..The 8 full papers presented in this book together with 1 short paper and 3 invited papers were carefully reviewed and selected from 13 submissions. They cover topics such as: modeling and analysis of metabolic, signaling, and genetic regulatory networks in living cells; models of tissues, organs, physiological models; models and methods coping with incomplete, uncertain and heterogeneous information including
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