Titlebook: Hepatic Stem Cells; Methods and Protocol Naoki Tanimizu Book 2019 Springer Science+Business Media, LLC, part of Springer Nature 2019 biliar

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Isolation of Bipotential Liver Progenitor Cells from Neonatal Mouse Liverpo pathways have been implicated in lineage determination of LPCs during development and regeneration. However, the molecular mechanisms governing the lineage specification have not been fully elucidated, yet. Epithelial adhesion molecule (EpCAM) is a marker of cholangiocytes and of LPCs. We found t

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Identification and Isolation of Clonogenic Cholangiocyte in Mouseally proliferating and differentiating cholangiocytes both in vitro and in vivo. In this protocol, we describe the method for isolation of primary cholangiocytes from mouse. To study the heterogeneity of cholangiocytes, we used flow cytometry-based cell sorting to isolate different subsets of cholan

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Isolation and Expansion of Rat Hepatocytic Progenitor Cellss a subpopulation of mature hepatocytes in a healthy liver. SHs can proliferate to form colonies in serum-free medium on hyaluronic acid-coated dishes, of which cells show CD44 positivity (CD44. SHs). CD44. SHs can be separated and purified from colonies by using anti-CD44 antibodies after enzymatic

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Specific Labeling and Lineage Tracing of Periportal Hepatocytes Using Two-Step Genetic Recombinationl agents. Although hepatocytes have been proposed to be the main source of new hepatocytes during regeneration, the existence of specialized liver stem cells has been long debated. In mice, oval cells or ductal cells have been postulated as such stem/progenitor pool. Exhaustive works from different

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Analysis for the Heterogeneity of Liver Progenitor Cells apparent by various experimental approaches, and here we describe a series of techniques to investigate the proliferative heterogeneity of these cells. We have developed a new technique by combining genetic lineage tracking and three-dimensional imaging methods. The data obtained can be used in sta

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Chemical Screening Using a Zebrafish Model for Liver Progenitor Cell-Driven Liver RegenerationLPCs). The zebrafish liver injury model is an important system to elucidate the molecular mechanisms underlying LPC-driven liver regeneration. Here, we describe a chemical screening method using the zebrafish model for identifying small molecules that can modulate LPC-driven liver regeneration.

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