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Titlebook: Virus-Induced Gene Silencing; Methods and Protocol Annette Becker Book 2013 Springer Science+Business Media New York 2013 VIGS.developmenta

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Rationale for Developing New Virus Vectors to Analyze Gene Function in Grasses Through Virus-Induce of genes during pathogen challenge and plant development. It also can be used in moderate-throughput reverse genetics screens to determine gene function. However, the number of viruses modified to serve as silencing vectors in grasses is limited, and the silencing phenotype induced by these vectors
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Utilizing Virus-Induced Gene Silencing for the Functional Characterization of Maize Genes During Inonditions that allow successful .. . infection of BMV pre-infected maize plants. This setup enables quantification of VIGS and its impact on .. . infection using a quantitative real-time PCR (q(RT)-PCR)-based readout.
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Book 2013non-model plants. Also included in this book are recently developed protocols for VIGS-derived microRNA production in the plant or protein over expression, as well as chapters devoted to summarizing the molecular mechanisms of VIGS action and the vector systems developed so far. Written in the succe
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Ivan Galis,Meredith C. Schuman,Klaus Gase,Christian Hettenhausen,Markus Hartl,Son T. Dinh,Jianqiang
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Ben Spitzer-Rimon,Alon Cna’ani,Alexander Vainstein
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