书目名称 | Virus-Induced Gene Silencing |
副标题 | Methods and Protocol |
编辑 | Annette Becker |
视频video | |
概述 | Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts.Includes supplementary materia |
丛书名称 | Methods in Molecular Biology |
图书封面 |  |
描述 | .Plants are amazing organisms to study, some are important sources for pharmaceuticals, and others can help to elucidate molecular mechanisms required for a plant’s development and its interactions with the biotic or abiotic environment. Functional genomics is vastly lagging behind the speed of genome sequencing as high-throughput gene function assays are difficult to design, specifically for non-model plants. Bioinformatics tools are useful for gene identification and annotation but are of limited value for predictions concerning gene functions as gene functions are uncovered best by experimental approaches. Virus-Induced-Gene-Silencing (VIGS) is an easy to use, fast, and reliable method to achieve down regulation of target gene expression.. Virus-Induced Gene Silencing: Methods and Protocols .provides detailed protocols for VIGS experiments in several plant species including model and non-model plants. Also included in this book are recently developed protocols for VIGS-derived microRNA production in the plant or protein over expression, as well as chapters devoted to summarizing the molecular mechanisms of VIGS action and the vector systems developed so far. Written in the succe |
出版日期 | Book 2013 |
关键词 | VIGS; developmental biology; genome sequencing; phenotypes; plant pathogens; plant research; gene expressi |
版次 | 1 |
doi | https://doi.org/10.1007/978-1-62703-278-0 |
isbn_softcover | 978-1-4939-5942-6 |
isbn_ebook | 978-1-62703-278-0Series ISSN 1064-3745 Series E-ISSN 1940-6029 |
issn_series | 1064-3745 |
copyright | Springer Science+Business Media New York 2013 |