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Titlebook: Urothelial Carcinoma; Methods and Protocol Wolfgang A Schulz,Michèle J. Hoffmann,Günter Niegi Book 2018 Springer Science+Business Media LLC

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Analysis of Chromosomal Alterations in Urothelial Carcinomaoma. By a conventional chromosome analysis on fresh biopsies, it is possible to delineate the status of ploidy and rough chromosomal aberrations. The multi-target fluorescence in situ hybridization (FISH) UroVysion test, for the rapid detection of chromosomal aneusomy of chromosomes 3, 7, and 17 and
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Immunohistochemical Analysis of Urothelial Carcinoma Tissues for Proliferation and Differentiation Mantibodies are bound to cellular antigens, captured by secondary antibodies or polymers, linked to enzymes and visualized by chromogenic substrates. Here, we describe an automated staining technique for larger slide batches as well as a manual protocol for only few slides using a polymer technique.
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Molecular Subtype Profiling of Urothelial Carcinoma Using a Subtype-Specific Immunohistochemistry Pacle invasive (MI) tumors. For analysis of NMI tumors, as few as two markers are needed, although classification is dependent also on pathological grade and histological evaluation. The result is a classification into the three tumor-cell phenotypes of NMI-BC, Urothelial-like (Uro), Genomically Unsta
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Defining the Pathways of Urogenital Schistosomiasis-Associated Urothelial Carcinogenesis through Trathe development of bladder cancer are poorly understood, mainly because of a dearth of tractable mouse models. We developed a mouse model of urogenital schistosomiasis through intramural injection of parasite eggs into the bladder wall to mimic the trapping of parasite eggs in the bladder. This appr
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Urothelial Carcinoma Stem Cells: Current Concepts, Controversies, and Methodstionally diverse cancer cells with a limited lifespan. To purify cancer stem cells, two basic approaches can be applied, the marker-based approach employing various more of less-specific cell surface marker molecules and a marker-free approach largely based on various self-protection mechanisms. Wit
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