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Titlebook: Urothelial Carcinoma; Methods and Protocol Wolfgang A Schulz,Michèle J. Hoffmann,Günter Niegi Book 2018 Springer Science+Business Media LLC

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https://doi.org/10.1007/978-1-4939-7234-0urinary; bladder; next generation sequencing techniques; carcinomas; immunotherapy
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Detection of APOBEC3 Proteins and Catalytic Activity in Urothelial CarcinomaBEC3B (A3B), and APOBEC3G (A3G) proteins is particularly challenging due to the large sequence homology of these proteins and limited availability of antibodies. Here we combine independent immunoblotting with an in vitro activity assay technique, to detect and categorize specific A3s expressed in urothelial bladder cancer and other cancer cells.
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Patient-Derived Bladder Cancer Xenograftse models retain the histological and genetic characteristics of the primary tumor and are considered a valuable platform for translational cancer research. This chapter describes the methodology to establish and propagate bladder cancer PDX model.
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978-1-4939-8425-1Springer Science+Business Media LLC 2018
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Urothelial Carcinoma978-1-4939-7234-0Series ISSN 1064-3745 Series E-ISSN 1940-6029
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Book 2018iding known pitfalls...Authoritative and practical, .Urothelial Carcinoma: Methods and Protocols. hopes that the techniques described in this volume will contribute to the current upturn in research on urothelial carcinoma and to the application of its results in clinical practice..
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