Titlebook: Synthetic Promoters; Methods and Protocol Mario Andrea Marchisio Book 2024 The Editor(s) (if applicable) and The Author(s), under exclusive

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Promoters in ,: A Toolbox for Fine-Tuned Gene Expressionvelopments that will enhance promoter design for .. Overall, this comprehensive overview underscores the importance of promoter choice and engineering for fully harnessing . biotechnological potential.

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Synthetic Promoter Design and Functional Evaluation in al promoters often have limited transcriptional capacity and thus fall short of the metabolic engineering requirements. This chapter provides protocols and guidelines for constructing and evaluating synthetic promoters in .. Moreover, these protocols are applicable for creating and testing various synthetic promoters in other host systems.

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dermatomyositis

Promoter Prediction in , Strain C58 by Using Artificial Intelligence Strategies gene expression depends on being able to identify promoters, because they are the most important component of gene expression. . (.) strain C58 was the subject of this study with the goal of creating a machine learning-based model to predict promoters. In this study, nucleotide density (ND), .-mer,

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accrete

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Hybrid Synthetic Promoters in , Built on Foreign Promoter Sequencess. Here, we describe a new design that makes use of the core promoters from foreign organisms: viruses, humans, and the yeast .. With this approach, we realized a library of 59 new constitutive promoters that span over nine folds in gene expression.

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CRISPR/Cas9-Mediated Promoter Engineering in echnologies have been proved to be efficient tools for genome editing in actinomycetes, making it easier and more efficient to perform gene insertion and substitution in actinomycetes in a scarless manner. In this chapter, we describe a routine protocol for CRISPR/Cas9-mediated promoter engineering