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Titlebook: Synthetic Promoters; Methods and Protocol Mario Andrea Marchisio Book 2024 The Editor(s) (if applicable) and The Author(s), under exclusive

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书目名称Synthetic Promoters
副标题Methods and Protocol
编辑Mario Andrea Marchisio
视频video
概述Includes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Synthetic Promoters; Methods and Protocol Mario Andrea Marchisio Book 2024 The Editor(s) (if applicable) and The Author(s), under exclusive
描述.This detailed volume presents numerous methods used in bioengineering to create new synthetic promoters in various organisms. Additionally, the book explores mathematical models, novel screening and construction techniques, modulation of protein expression, and various applications ranging from biosensors to viral resistance. Written for the highly successful .Methods in Molecular Biology. series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls... ..Authoritative and practical, .Synthetic Promoters: Methods and Protocols. offers researchers a guide to the latest advances in this branch of synthetic biology..
出版日期Book 2024
关键词Gene circuit components; Bioengineering; Random mutation; Hybrid sequence assembly; CRISPR-Cas systems; P
版次1
doihttps://doi.org/10.1007/978-1-0716-4063-0
isbn_softcover978-1-0716-4065-4
isbn_ebook978-1-0716-4063-0Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightThe Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines
The information of publication is updating

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Sara Yousefi Taemeh,Nima Dehdilani,Lena Goshayeshi,Hesam Dehghani of ‘them-versus-us’ thinking which both offers the feelings of security and of belonging to a group and at the same time invites the risk of dehumanizing and excluding the other? Socialization from this perspective is a common978-94-6209-092-7
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Promoters and Synthetic Promoters in tive promoters, the impact of different promoters on both native and foreign protein expression, the discovery of fresh promoters, and strategies conducive to future research aimed at refining and enhancing protein expression in .. Characterizing new promoters and adopting innovative expression syst
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Optimizing Rice Genomics: Employing the Hypercompact Cas12j2 System for Targeted Transcriptional Regve ventured beyond mere gene editing to develop a Cas12j2-based approach for targeted epigenome editing, particularly in the context of DNA methylation. This was demonstrated through the targeted methylation of the . promoter, as verified by Next-Generation Sequencing of bisulfite sequencing PCR pro
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Exploring the Function of Gene Promoter Regulatory Elements Using CRISPR Tools a specific focus on the use of CRISPR technology on enhancers and silencer elements of the . gene promoter. We explain and discuss processes for the deletion of/interference with regulatory elements within the promoter, employing CRISPR-based approaches. Furthermore, we demonstrate that a CRISPR/Ca
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Fine-Tuning Gene Expression in Bacteria by Synthetic Promotersc promoters for the cyanobacteria, which are phototrophic microorganisms, are evaluated, especially using the model cyanobacterium . sp. PCC 6803. Moreover, a variety of algorithms based on machine learning methods were developed to characterize the features of promoter elements. Some of these in si
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Tuning of Protein Expression by Promoter Engineeringdetermined. As the strength of the promoter of Est PS decreases, the expression level also decreases (from 100% to 10%). Conversely, the TAIC expression level is increased. This developed system produced a higher sitagliptin intermediate than enzymes’ unoptimized expression level.
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roups. Answers to the second question have proven much more difficult to find, although the question has caught the imagination of naturalists and scientists alike for centuries. Somehow, the individuals in the group seem to act in unison. They turn together, they flow around obstacles, and they mov
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