Titlebook: Steroid Receptors; Methods and Protocol Gabriella Castoria,Ferdinando Auricchio Book 2014 Springer Science+Business Media New York 2014 chr

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,Analysis of Chromatin–Nuclear Receptor Interactions by Laser-Chromatin Immunoprecipitation,interactions, and specifically hormone-triggered DNA–nuclear receptor interactions, are key events which are still poorly understood. To date, the most commonly used approach in studying chromatin interactions is the immunoprecipitation of chemically cross-linked chromatin (ChIP) coupled with single

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Paxillin and Steroid Signaling: From Frog to Human,ell motility. In addition to its structural functions, paxillin has more recently been shown to function as a regulator of cell division—mediating steroid-triggered meiosis in oocytes as well as steroid- and growth factor-induced proliferation in prostate and breast cancer. Paxillin mediates these p

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Multi-well Plate Immunoassays for Measuring Signaling Protein Activations/Deactivations and Membranoblot scanning. The assays make use of the now abundant antibodies designed to recognize receptor subtypes and posttranslationally modified signaling proteins. By optimizing permeabilization and fixation conditions, mainly based on specific cell types, the assay can be adapted to the study of many d

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