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Titlebook: Steroid Receptors; Methods and Protocol Gabriella Castoria,Ferdinando Auricchio Book 2014 Springer Science+Business Media New York 2014 chr

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书目名称Steroid Receptors
副标题Methods and Protocol
编辑Gabriella Castoria,Ferdinando Auricchio
视频video
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Steroid Receptors; Methods and Protocol Gabriella Castoria,Ferdinando Auricchio Book 2014 Springer Science+Business Media New York 2014 chr
描述.Steroid Receptors: Methods and Protocols. presents a selection of techniques that have been recently applied to the analysis of steroid receptors, powerful tools for the advancement of our understanding of both the mechanisms regulating gene transcription and the rapid signaling responses of tissues to signals. Research in this area has generated a wealth of data allowing the elucidation of steroid receptor mechanisms and improving the treatment of many endocrine disorders, above all cancers. Chapters cover methods to analyze gene transcription, chromatin and proteomic modifications, extra-nuclear signaling regulation, development of cell and animal models, and preparation of new antibodies. Written in the successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls..Authoritative and easily accessible, .Steroid Receptors: Methods and Protocols. offers an updated view on a variety of modern methods that will hopefully contribute to improving our knowledge on the integration of ste
出版日期Book 2014
关键词chromatin; gene transcription; proteomic modifications; steroid receptors; steroid signaling
版次1
doihttps://doi.org/10.1007/978-1-4939-1346-6
isbn_softcover978-1-4939-4845-1
isbn_ebook978-1-4939-1346-6Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media New York 2014
The information of publication is updating

书目名称Steroid Receptors影响因子(影响力)




书目名称Steroid Receptors影响因子(影响力)学科排名




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书目名称Steroid Receptors读者反馈学科排名




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Multi-well Plate Immunoassays for Measuring Signaling Protein Activations/Deactivations and Membranproteins. By optimizing permeabilization and fixation conditions, mainly based on specific cell types, the assay can be adapted to the study of many different antigens of importance to hormonal and neurotransmitter signaling scenarios.
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Rapid Estrogen Effects on Aromatase Phosphorylation in Breast Cancer Cells,e describe a novel assay protocol to detect changes in the phosphorylation status of the purified six tandem histidine-tagged form of human aromatase transiently expressed in breast cancer cells. This method overcomes the limitations of current immunoprecipitation techniques performed using commercial available aromatase antibodies.
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