Titlebook: Stem Cell Renewal and Cell-Cell Communication; Methods and Protocol Kursad Turksen Book 2015 Springer Science+Business Media New York 2015

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Multiplex Immunoassays for Quantification of Cytokines, Growth Factors, and Other Proteins in Stem iques are powerful for analyzing growth factors, trophic factors, angiogenic factors, hormones, cytokines, chemokines, soluble receptors, and other proteins which play key roles in intercellular communication and operate as potent regulators of stem cell survival, proliferation, differentiation, or

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Porous Membrane Culture Method for Expansion of Human Pluripotent Stem Cells,se of animal feeder cells and enzyme treatments used in conventional hPSC culture systems. Here, we describe a novel culture method using a porous membrane that allows to maintain stable attachment and expansion of hPSCs, obviates the need for enzyme treatment, and also reduces feeder layer contamin

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Generation, Expansion, and Differentiation of Human Pluripotent Stem Cell (hPSC) Derived Neural Proand subsequent differentiation into the various cell types that comprise the central nervous system (CNS), could provide an unlimited source of cells for neural-related cell-based therapies and disease modeling. However, the use of NPCs for the study and treatment of a variety of debilitating neurol

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Isolation, Long-Term Expansion, and Differentiation of Murine Neural Stem Cells,s. Here, we describe some methods for the isolation of neural stem cells (NSCs) from the adult murine subependymal zone (SEZ), their extensive culturing and the assessment of their full developmental potential, particularly with respect to their differentiation capacity. The procedure includes chemi

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Generation, Expansion, and Differentiation of Cardiovascular Progenitor Cells from Human Pluripotential source for the study of early embryonic cardiovascular development and stem cell-based therapies for congenital and acquired heart diseases. To fully realize their values, it is essential to establish an efficient and stable differentiation system for the induction of these pluripotent stem cel