Titlebook: Stem Cell Renewal and Cell-Cell Communication; Methods and Protocol Kursad Turksen Book 2015 Springer Science+Business Media New York 2015

hypnotic

Ascendancy

A Human Colonic Crypt Culture System to Study Regulation of Stem Cell-Driven Tissue Renewal and Phy and transport of nutrients and fluid. Investigation of gut epithelial physiology in health and disease has been hampered by the lack of ex vivo models of the native human intestinal epithelium. Recently, remarkable progress has been made in defining intestinal stem cells and in generating intestina

离开真充足

Nomadic

condone

JADED

Electrophysiological Recordings from Neuroepithelial Stem Cells,ontact with the basement membrane of the neuroepithelium. Under such circumstances, intracellular recording with a fine-tipped microelectrode is a successful method to study the electrophysiological properties of the neuroepithelial stem cell. This chapter describes the detailed techniques of intrac

脱水

In Vivo Stem Cell Transplantation Using Reduced Cell Numbers,entifying the potential of DPSCs to function as stem cells. Previously reported DPSC transplantation methods have used with huge numbers of cells, along with hydroxyapatite/tricalcium phosphate (HA/TCP), gelatin and fibrin, and collagen scaffolds. This protocol describe a transplantation protocol th

FRONT

Desert

Generation, Expansion, and Differentiation of Cardiovascular Progenitor Cells from Human Pluripotenls (PSCs) into the CVPCs and robustly expand them in culture, and then further differentiate these CVPCs into multiple cardiovascular cell types. Here we describe the protocols for efficient derivation, expansion, and differentiation of CVPCs from hPSCs in a chemically defined medium under feeder- and serum-free culture conditions.

WITH