Titlebook: Spatial Genome Organization; Methods and Protocol Tom Sexton Book 2022 The Editor(s) (if applicable) and The Author(s), under exclusive lic

correspondent

The High-Salt Recovered Sequence-Sequencing (HRS-seq) Method: Exploring Genome Association with Nuclation, like nuclear bodies, are crucial to regulate genome functions, and in particular transcription. Here we describe the protocol of the high-salt recovered sequence sequencing method whose principle relies on high-throughput sequencing of genomic DNA trapped into large RNP complexes that are mad

凝视

ear-canal

Simultaneous Quantification of Spatial Genome Positioning and Transcriptomics in Single Cells with scification. Yet it is still an outstanding question in the field what the direct impact of spatial genome organization on gene expression is. To elucidate this relationship further, we have recently developed scDam&T-seq, a method that simultaneously quantifies protein–DNA interactions and transcrip

是限制

High-Throughput DNA FISH (hiFISH)nd analysis to visualize and localize tens to hundreds of genomic loci in up to millions of cells. hiFISH can be used to measure physical distances between pairs of genomic loci, radial distances from genomic loci to the nuclear edge or center, and distances between genomic loci and nuclear structur

palliate

慷慨不好

提名的名单

projectile

pus840

High-Throughput DNA FISH (hiFISH)FISH to measure distances between genomic loci, including all steps involved in DNA FISH probe design and preparation, cell culture, DNA FISH staining in 384-well imaging plates, automated image acquisition and analysis, and, finally, statistical analysis.

Landlocked

Assessment of Multiway Interactions with Tri-C investigate how multiple .-regulatory elements interact together in higher-order structures in single nuclei and address questions regarding structural cooperation between these elements. Here, we describe the procedure for designing and performing a Tri-C experiment.