Titlebook: Skin Stem Cells; Methods and Protocol Kursad Turksen Book 2024Latest edition The Editor(s) (if applicable) and The Author(s), under exclusi

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Flow Cytometric and Immunohistochemical Follow-Up of Spermatogonial Lineage Commitment,croenvironment and predicting progress of spermatogenesis. Here we define the evaluation of mouse male germ cell microenvironment at single cell and whole tissue section levels by using flow cytometric and immunohistochemical approaches.

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1064-3745 experts.This updated edition explores techniques in identifying and characterizing various skin stem cells and generating skin-like structures in vitro. The goal of the book is to present exciting advances in skin biology in an effort to provide a better understanding of skin and, eventually, to al

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Isolation, Characterization, and Utilization of Human Skin Basal and Suprabasal Epidermal Stem Cellcompositions. We describe as useful technique the histological analysis of tissue sections. We further illustrate the biological characterization of isolated and cultured basal and suprabasal interfollicular keratinocytes by cell sorting, cytospin immunostaining, colony forming efficiency, and long-term dermo-epidermal organotypic cultures.

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Isolation of Human Progenitor Epidermal Cells on Collagen Type IV and Analysis of Their Markers wit incubation with dispase followed by cell isolation for epidermis by digestion with trypsin. Cell isolated with this method can be seeded on collagen IV-coated dishes. The methods of analysis of epidermal stem cells markers (e.g., CD71, CD29) with flow cytometry and RT-PCR are also included.

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Characterization of the Newborn Epidermis and Adult Hair Follicles Using Whole-Mount Immunofluoreschair follicles that invaginate into the dermis. In adult skin, the hair follicle undergoes cyclic regeneration fueled by hair follicle stem cells located in the bulge. Three-dimensional and high-resolution imaging of these structures using whole-mount immunofluorescent staining allows to better characterize epidermal progenitors and stem cells.