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Titlebook: Skin Stem Cells; Methods and Protocol Kursad Turksen Book 2024Latest edition The Editor(s) (if applicable) and The Author(s), under exclusi

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楼主: Harding
发表于 2025-3-25 04:13:40 | 显示全部楼层
,Human-Induced Pluripotent Stem Cell‒Derived Keratinocytes, as Therapeutic Option in Vitiligo,pathologic destruction of melanocytes, which produce melanin. Research has focused on the abnormalities of melanocytes and their interaction with neighboring keratinocytes. Current treatments are mainly immunosuppressive drugs and UV radiation, which are scarce and ineffective. To treat vitiligo, re
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GMP-Based Isolation of Full-Term Human Placenta-Derived NK Cells for CAR-NK Cell Therapy in Malignathe high immunogenicity of melanoma renders it amenable to immune therapy, and NK cells have been identified as possessing anti-tumor properties in immunotherapy. The development of chimeric antigen receptor (CAR)-modified NK cells, or CAR-NK cells, has shown potential in enhancing immunotherapeutic
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Preparation and Validation of Zebrafish Psoriasis Model to Investigate the Therapeutic Effects of Sstinal eruptions, and sometimes arthritis. Moreover, most of the psoriatic subjects report life challenges due to the condition, impacting social activities and daily tasks. Generally, psoriasis treatment options depend on the severity, coexisting conditions, and medical availability. Although psori
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Development of CAR-NK Cells Targeting cSCC-Specific Antigens for Precision Immunotherapy,ehavior, frequent poor response to standard therapy, and capacity to metastasize to distant areas. Utilizing the body’s natural immune defense mechanisms, particularly through the use of chimeric antigen receptor (CAR) technology, is receiving increasing attention in the dynamic field of oncological
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Application of CRISPR/Cas9 Pooled Screening for a Non-immediate Readout Model, provides an invaluable alternative to classical and RNAi-based screening methods. Combined with high-throughput sequencing and bioinformatics, CRISPR-/Cas9-based pooled screening methods provide unbiased and robust data. In this protocol, we employed CRISPR-/Cas9-based pooled screening for a non-binary and non-immediate readout.
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Preparation and Use of shRNA for Knocking Down Specific Genes,shRNA templates for at least 10 different genes and confirmed them by dideoxy sequencing. The knockdown of 75–90% for two mRNA expressing genes, . and keratin ., and a long non-coding RNA, ., is presented here.
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